Coding

Part:BBa_K2969004:Design

Designed by: Ziyi Zhao   Group: iGEM19_UCAS-China   (2019-09-01)


TEVts17#


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 319
    Illegal SapI.rc site found at 667


Design Notes

No


Source

Zheng, Y., Meng, F., Zhu, Z., Wei, W., Sun, Z., Chen, J., . . . Chen, G.-Q. (2019). A tight cold-inducible switch built by coupling thermosensitive transcriptional and proteolytic regulatory parts. Nucleic Acids Research. doi:10.1093/nar/gkz785 In this paper, the author combined the thermosensitive proteolytic with transcriptional regulatory parts to build a tight cold-inducible switch and put it into various applications.

References

Zheng, Y., Meng, F., Zhu, Z., Wei, W., Sun, Z., Chen, J., . . . Chen, G.-Q. (2019). A tight cold-inducible switch built by coupling thermosensitive transcriptional and proteolytic regulatory parts. Nucleic Acids Research. doi:10.1093/nar/gkz785 In this paper, the author combined the thermosensitive proteolytic with transcriptional regulatory parts to build a tight cold-inducible switch and put it into various applications.